FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414373

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414373
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1957272
Sequences flagged as poor quality0
Sequence length61
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30960.15817934349441468No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT19950.033.6392751
TGGTATC20250.032.3086282
GTATCAA70650.026.433661
TATCAAC77300.024.00442
TTAACGG1900.023.16028435
ATCAACG82550.022.4071643
AGAGTAC64750.022.3845511
TCAACGC82350.022.3280834
CAGAGTA65900.022.07739310
CAACGCA83600.021.9942285
AACGCAG84050.021.9435566
GGTATCA37100.020.4613781
ACGCAGA92750.019.8566177
TAACGGC2157.2759576E-1219.18802636
CGCAGAG96650.018.944518
CGCCTAG750.00740710118.3361641
AGTACGG79200.017.98796813
GCAGAGT102050.017.9457249
GAGTACG81700.017.5385312
TTTAACG2555.456968E-1217.255834
Produced by FastQC (version 0.11.5)