FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414375

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414375
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2420110
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG45160.18660308828937527No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAA36020.14883620992434227No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGC32270.1333410464813583No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTT30550.12623393151550963No Hit
GTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACAGGGAGG26720.11040820458574198No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT25520.10544975228398709No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT25320.10462334356702795No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC67300.032.0492132
GTGGTAT71150.030.4110851
GACAGGT8150.027.999651
GCTTGTA9250.027.0477851
GTATCAA219700.025.779191
TTGTACG2600.025.3588093
AGAGTAC160450.023.43319911
CAGAGTA162700.023.1419910
CTTGTAT5600.022.5700512
AACGCAG249050.022.5040916
TATCAAC254000.022.284162
GCTTGGG4250.021.9949041
TCAACGC260650.021.5117724
GCACCGC9800.021.32908810
ATCAACG264950.021.214493
CAACGCA265400.021.1672975
GCTTGTC4750.020.8372781
AGTCCGG2800.020.6291854
TGGACGC3000.020.1615830
ACGCAGA280000.020.0603947
Produced by FastQC (version 0.11.5)