FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414379

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414379
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1816893
Sequences flagged as poor quality0
Sequence length61
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27990.1540542013206061No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT32350.038.8557661
TGGTATC32100.038.628312
GTATCAA115550.026.136411
AGAGTAC84400.024.21096611
CAGAGTA88050.023.581510
TATCAAC127100.023.5351832
GGTATCA61400.022.9358671
TCAACGC136650.021.867264
ATCAACG137600.021.7362633
AACGCAG139700.021.3898436
CAACGCA140100.021.3680155
ACGCAGA156050.019.1503227
CGCAGAG157150.018.9993068
AGTACGG109100.018.6792413
GAGTACG109750.018.59367212
GCAGAGT163400.018.2756049
GCCGTTA1403.3205579E-617.67991615
CGGGTCA6300.017.46164717
GCTTGTA6000.017.4199121
TTAAACG2403.8198777E-1117.18928122
Produced by FastQC (version 0.11.5)