FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2012866
Sequences flagged as poor quality0
Sequence length91
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT108800.54052281672004No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77970.38735812518071244No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54190.2692181198351008No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA48970.24328494792996655No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC36000.1788494614147191No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT34670.1722419674235642No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATG29170.144917744151871No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT28550.14183755898306197No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCA28210.14014842518081183No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATA26600.132149879823098No Hit
CTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAA25020.1243003756832298No Hit
GTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAG24670.12256156147503112No Hit
GTATCAACGCAGAGTACGGGACTATCACCCTCTACGGTAGCACTTCCCAG24370.12107114929657513No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGA24340.12092210807872954No Hit
GTATCAACGCAGAGTACGGGGTCAAGTAATTAAGTGCATGTGGTGGATGC24080.11963041752406768No Hit
ATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAGTACCC22750.11302292353291277No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAA22490.11173123297825091No Hit
GGGTACTGAGATGTTTCACTTCCCCTCGTTCGCCTCGCATGACTATGTAT21850.10855168699754479No Hit
GTACTGAGATGTTTCACTTCCCCTCGTTCGCCTCGCATGACTATGTATTC20140.10005633758034564No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT30050.053.3253861
TGGTATC30450.052.894872
GTATCAA113750.041.2529341
GGTATCA60650.037.5639421
TATCAAC131500.035.872272
TCAACGC137250.034.1492844
ATCAACG138950.033.7620663
CAACGCA139850.033.5751765
AACGCAG142350.033.015376
CGCAGAG155350.030.224478
ACGCAGA155750.030.174137
GCAGAGT163700.028.8132869
CAGAGTA100000.025.66583410-11
GAGTACG112050.022.94390912-13
AGAGTAC97200.022.57992710-11
GACAGGT10000.022.1024531
AGTACGG110050.019.86640212-13
ACGGGAT14800.019.52385916-17
GTACGGG130700.019.4588814-15
CCGTCTT9350.018.4122884-85
Produced by FastQC (version 0.11.5)