FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414381

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414381
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2012866
Sequences flagged as poor quality0
Sequence length61
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT42630.21178757055859654No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT33150.16469054571938718No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC32800.16295173151118852No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCC27480.13652175554656892No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAGCTTCGGGGA25910.1287219318126492No Hit
GTATCAACGCAGAGTACGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCA25320.1257907878616858No Hit
GTATCAACGCAGAGTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCT24890.12365453040589887No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGATATTTGCCGCCT24120.1198291391478618No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCT23900.11873617021699408No Hit
CTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCC23400.11625214991956744No Hit
GTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAG22940.11396685124593489No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAA21690.10775680050236827No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAA21160.10512373898709601No Hit
GGGTACTGAGATGTTTCACTTCCCCTCGTTCGCCTCGCATGACTATGTATTCATCATGCGA20380.10124866732311043No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT18950.036.2839161
TGGTATC18650.036.2668722
GTATCAA91150.029.5097481
GGTATCA42850.026.9576361
TATCAAC104950.025.8838162
ATCAACG110750.024.2007243
TCAACGC110800.024.161394
AGAGTAC104600.024.05215611
AACGCAG112550.023.9387786
CAACGCA113150.023.8088825
CAGAGTA106600.023.677110
CGCAGAG115800.023.1945448
ACGCAGA116950.023.0604937
GCAGAGT121050.022.2129529
TAACGGC1701.2551027E-1021.03343436
AGTACGG118350.020.95885813
GAGTACG119850.020.8097212
TACGGGG45050.018.49643315
CGATCGT1352.3480534E-618.33364947
GTACGGG137200.017.89935914
Produced by FastQC (version 0.11.5)