FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414387

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414387
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1391278
Sequences flagged as poor quality0
Sequence length61
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22530.16193744169030203No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT24100.039.244591
TGGTATC23700.038.9676742
GTATCAA78700.024.3847961
TACGCGA952.316594E-623.15786629
AGAGTAC56900.022.81274211
CAGAGTA58950.022.34598210
TATCAAC86450.022.2241782
GGTATCA43750.021.923713
ATCAACG93350.020.579253
TCAACGC93750.020.5207614
CAACGCA95600.020.1236535
AACGCAG96650.019.962626
GCTTGTA4750.019.679941
ACGCAGA106300.018.1264977
CGCAGAG106850.017.9077858
GTACGCG1403.3184588E-617.68045628
GAGTACG74500.017.38650912
AGTACGG73500.017.32372913
GCAGAGT112200.017.0079469
CGGGAAA4650.016.55971317
Produced by FastQC (version 0.11.5)