FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414390

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414390
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences862041
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT51340.5955633200741033No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42000.48721580528072334No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT38150.44255435646332364No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26660.3092660325900972No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11780.1366524330049267No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCC10500.12180395132018083No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8810.10219931534578983No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT12450.062.4775541
TGGTATC13100.060.0194932
GTATCAA48550.037.558681
GGTATCA27550.036.873861
TATCAAC53100.034.256352
TCGACGT500.002790094833.9962849
ATCAACG57550.031.605683
TCAACGC58550.030.993294
CAACGCA59100.030.7048575
AACGCAG61200.029.8595836
CGCAGAG67150.027.0207968
ACGCAGA68200.026.7917087
GCAGAGT68850.026.3551469
CAGAGTA38000.026.05636410-11
CGACTCT1159.1745915E-625.8682385
GAGTACG43200.022.87076612-13
AGAGTAC36750.020.92968610-11
GTACGGG47350.020.8213814-15
ATACGGT1050.00418705820.2370596
ACGGGAT8500.018.24694816-17
Produced by FastQC (version 0.11.5)