FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414391

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414391
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences862041
Sequences flagged as poor quality0
Sequence length61
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG78430.9098175144801697No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT7950.037.729321
TGGTATC8150.037.4526442
CGTACGA553.489312E-530.00066635
GTATCAA38250.029.784381
TCCGATA507.034342E-427.49741723
TATCAAC42700.027.1770112
GGTATCA18000.026.4480231
AGAGTAC40350.025.5552211
CAGAGTA41200.025.0947310
TGCGTCG550.001226976924.9976529
TGGACCG550.001228222624.9933035
ATCAACG45900.024.8557323
TCAACGC46300.024.581624
CAACGCA47000.024.275415
AACGCAG48300.023.6803286
CGGGGAA2750.023.0005117
TAGACGT600.002038980122.9091974
CGCAGAG50050.022.7437978
TACGGGG19000.022.57944915
ACGCAGA50850.022.4928177
Produced by FastQC (version 0.11.5)