FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414393

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414393
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1140099
Sequences flagged as poor quality0
Sequence length61
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17520.15367086542484468No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT11630.1020086852106703No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAA11520.10104385671770609No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT20700.039.0468141
TGGTATC21250.038.4175722
GTATCAA75000.025.2194631
AGAGTAC56050.023.84529711
CAGAGTA56950.023.70990610
TATCAAC81950.022.9759772
CGATCGT852.565512E-522.6525247
GGTATCA41250.022.193661
ATCAACG87700.021.4354083
CGACGGT903.9949227E-521.38934910
TCAACGC87950.021.311984
AACGCAG88650.021.081696
CAACGCA89600.020.8888425
AGTACGG70100.018.98757213
ACGCAGA98650.018.9455017
GAGTACG70450.018.85420412
CGCAGAG100100.018.6751638
CCGCGCA750.007401549318.33775348
GCTTACC1503.1710806E-718.3280961
TATACTG2251.4551915E-1118.3224685
Produced by FastQC (version 0.11.5)