FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414398

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414398
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences726567
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22130.30458305978664046No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT20630.28393802636233134No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12880.17727202033673425No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAA11120.15304851445221157No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT10480.14423996685783966No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA10040.138184090386709No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT9340.12854974145536474No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC8760.12056699519796521No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8440.11616272140077928No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCA8420.11588745428845516No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT7720.10625310535711091No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT7690.10584020468862472No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC15000.040.2320632
GTATCAA54450.039.1222951
GTGGTAT16100.036.1808851
GACAGGT2400.035.432621
AACGCAG62550.034.175586
TATCAAC63700.034.1590462
TCAACGC65150.033.072634
ATCAACG66250.032.6518023
ACAGGTA3400.032.5146371
CAACGCA66800.032.319345
CAGGGTC550.004447629730.9081134
ACGCAGA70400.030.304447
CGCAGAG70750.030.0944568
GGTATCA29700.030.064041
GCTTGTA5250.029.1559831
GCAGAGT76950.027.6696919
GTCGAGG7600.026.8412598
GACAGGC1307.9899837E-726.1656271
GCCAGAT2950.024.4907538
CAGAGTA47650.023.4567610-11
Produced by FastQC (version 0.11.5)