FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414401

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414401
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences642144
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9500.14794189465291274No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAA9460.1473189814122689No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT8080.1258284746100563No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT6830.10636243583993621No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC6800.10589525090945334No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTCC501.817498E-533.0033217
TGGTATC15950.031.8798892
GTGGTAT16450.030.9108941
CCGGGCT400.00786672627.50276613
CGGGCCG400.00786672627.50276617
TCCGGGC400.00786672627.50276612
GTATCAA51700.024.827451
AGAGTAC38100.023.96566411
CAGAGTA38950.023.8663310
TTGTACG701.8058029E-423.5591163
GCTTGTA3150.023.5591131
AGCGAGC600.002036131722.9136168
TATCAAC56300.022.5059972
AACGCAG56900.022.2221016
TCAACGC58800.021.4556224
ATCAACG59400.021.2851723
GGGCCGC650.003237784621.15432518
GCTTGTC650.003248038721.1427961
CAACGCA60150.020.928385
AAACGCA1601.3333192E-920.62707324
Produced by FastQC (version 0.11.5)