FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414407

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414407
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1377766
Sequences flagged as poor quality0
Sequence length61
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG138831.0076457105197836No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA31050.028.7013551
GTGGTAT7000.027.8984031
GGTATCA14600.026.7518921
TATCAAC34450.025.5382142
TGGTATC7300.025.2337442
ATCAACG36300.023.9302463
CAACGCA36600.023.881715
TCAACGC36700.023.6694264
AACGCAG37450.023.3413646
AGAGTAC36950.022.69586211
CAGAGTA38500.022.13680610
ACGCAGA40050.021.8260697
CGCAGAG41400.021.379948
AGTACGG40600.020.38976113
GCAGAGT44750.019.7815849
TACGGGG19200.019.33767515
GAGTACG44250.018.70381712
GCCGTAT1051.3050054E-418.33697754
GTGTTAG1706.0001184E-817.7976551
GTACGGG45400.017.62823514
Produced by FastQC (version 0.11.5)