FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414409

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414409
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences574394
Sequences flagged as poor quality0
Sequence length61
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9480.16504350672186688No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGTA202.620424E-454.9663965
TGGTATC13600.038.6078072
GTGGTAT13900.038.1800651
GTACGGC350.00409622531.4312614
CGTGCGG350.00409797231.4285242
ATAGTCG350.004110219431.4093693
ATCGTCG400.0078666227.50235446
CGTATAC400.00789341327.4831983
CAGGACG400.00789341327.4831984
TAGTCGT507.0527557E-427.4831984
GTATCAA43500.025.411541
TTAAACG1201.0822987E-925.21268522
CTAGCCT550.001230182624.9847244
AGAGTAC29550.024.10317611
CAGAGTA30150.023.89714210
GGTATCA22900.023.7627643
TATCAAC47500.023.2076642
ATCAACG50450.021.8449213
TCAACGC50700.021.791414
AACGCAG51600.021.4131936
Produced by FastQC (version 0.11.5)