FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414410

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414410
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences777135
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT41700.5365863073983285No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31000.39890109183089173No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT29000.3731655375192212No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21050.2708667091303313No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8680.11169230571264965No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT11850.055.9607621
TGGTATC12100.054.0914842
GTATCAA41300.041.9940221
TATCAAC45050.038.6796532
ATCAACG48050.036.1739163
TCAACGC48100.036.136314
CAACGCA49050.035.5230645
AACGCAG50850.034.3491866
GGTATCA20650.033.965751
CGCAGAG56600.030.709488
ACGCAGA57550.030.2025477
GCAGAGT59200.029.3607529
CAGAGTA33000.026.33401110-11
AGCGTCA1351.115457E-625.1838323
AGAGTAC32100.023.49800510-11
GAGTACG38450.022.71188212-13
GCGTCAG1502.7894912E-622.665454
ACGGGAT7400.022.39600416-17
GTACGGG39150.022.08870514-15
TTACGTC1955.102811E-821.7937014
Produced by FastQC (version 0.11.5)