FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414411

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414411
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences777135
Sequences flagged as poor quality0
Sequence length61
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG57600.7411839641761084No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT6200.039.0592731
TGGTATC6950.034.8262982
GTATCAA33300.029.2544651
GTATAGA852.6886482E-829.1377961
GGTATCA15150.027.0649221
TATCAAC37900.026.0534172
AGAGTAC37500.024.93292411
CAGAGTA38400.024.27538310
ATCAACG40350.024.1244473
TCAACGC40650.023.8772244
CAACGCA41400.023.642395
AACGCAG42700.022.7923536
CGCAGAG43650.022.3607238
TGCCGAC3700.022.29836855
AGTACGG41100.022.21374913
ACGCAGA44500.021.8718247
GCAGAGT45900.021.2659789
CGGGATA1701.2551027E-1021.03041817
GAGTACG43550.020.96406412
TACGGGG18050.020.11176915
Produced by FastQC (version 0.11.5)