FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414412

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414412
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences889405
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51060.5740916680252528No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT50800.5711683653678583No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA34260.38520134247052806No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32330.36350144197525314No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC32330.36350144197525314No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT29320.3296585919800316No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATG26070.2931173087625997No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT25950.2917680921514945No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT24850.27940027321636374No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCA24580.2763645358413771No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGA23540.2646713252117989No Hit
ATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAGTACCC22590.25399002704054957No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT21300.2394859484711689No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATA20940.2354382986378534No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAA20330.2285797808647354No Hit
CTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAA20080.22576891292493298No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19020.21385083286017056No Hit
GTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAG19010.21373839814257845No Hit
GTATCAACGCAGAGTACGGGACTATCACCCTCTACGGTAGCACTTCCCAG18900.21250161624906538No Hit
CTTAATGGGCTGATCCCCGTTCGCTCGCCGCTACTGAGGGAATCTCAATT18820.2116021385083286No Hit
GGGTACTGAGATGTTTCACTTCCCCTCGTTCGCCTCGCATGACTATGTAT18100.20350683884169754No Hit
GTATCAACGCAGAGTACGGGGTCAAGTAATTAAGTGCATGTGGTGGATGC17910.2013705792074477No Hit
CATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGCTACTGAGGGAAT17810.2002462320315267No Hit
GTACTGAGATGTTTCACTTCCCCTCGTTCGCCTCGCATGACTATGTATTC16950.19057684631860625No Hit
GCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCTGAATGGGG16720.187990847813988No Hit
CCGCTACTGAGGGAATCTCAATTGATTTCTTTTCCTAAGGGTACTGAGAT16550.18607945761492234No Hit
CGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGC16380.18416806741585667No Hit
CCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTT16340.18371832854548828No Hit
ATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCA16200.18214424249919892No Hit
GGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGAGGGGAA15700.176522506619594No Hit
GTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCG14400.16190599333262126No Hit
GTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAGCTTCGGGGAGGCG14330.16111895030947657No Hit
TCTCAGTACCCTTAGGAAAAGAAATCAATTGAGATTCCCTCAGTAGCGGC14270.160444342003924No Hit
GTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAGTACCCTTAGGAA14130.1588702559576346No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13990.15729616991134523No Hit
CTACTGAGGGAATCTCAATTGATTTCTTTTCCTAAGGGTACTGAGATGTT13960.15695886575856893No Hit
GCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCTG13690.15392312838358227No Hit
GCAAATATCCTGTGATCCGGAGATTTCTGAATGGGGAAACCCACTTACCA13680.15381069366599018No Hit
TCGCATGACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGT12640.14211748303641197No Hit
GATGAATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAG12500.1405433969901226No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11920.13402218336978092No Hit
AATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACG11670.13121131542997846No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11510.1294123599485049No Hit
CCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAGCTTCG11470.12896262107813652No Hit
CCCACTTACCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAG11070.12446523237445259No Hit
TCCTAAGGGTACTGAGATGTTTCACTTCCCCTCGTTCGCCTCGCATGACT10720.12053001725872915No Hit
GTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAG10570.11884349649484767No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10470.1177191493189267No Hit
GTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCG10410.11704454101337412No Hit
TTTCTGAATGGGGAAACCCACTTACCATAAGGTAGGTATCGCATGATGAA10370.11659480214300572No Hit
GTATCAACGCAGAGTACGGGTCAAGTAATTAAGTGCATGTGGTGGATGCC9830.11052332739303242No Hit
CCCTTAGGAAAAGAAATCAATTGAGATTCCCTCAGTAGCGGCGAGCGAAC9380.10546376510138801No Hit
ATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCATTC9380.10546376510138801No Hit
GTATCGCATGATGAATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAA9270.10422698320787492No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9240.10388967905509863No Hit
GTGGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTT9190.10332750546713815No Hit
GATCCCCGTTCGCTCGCCGCTACTGAGGGAATCTCAATTGATTTCTTTTC9050.10175341942084877No Hit
CAGTAGCGGCGAGCGAACGGGGATCAGCCCATTAAGTTATATGTGTTTTA9030.10152854998566457No Hit
GAGTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCT9010.10130368055048039No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8960.10074150696251988No Hit
CTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATA8900.10006689865696732No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC28450.053.178872
GTGGTAT28950.050.6655651
GACAGGT3000.041.0978471
GTATCAA103250.037.512041
CGTCAGA1401.8189894E-1236.42715
TATCAAC116100.034.3719982
CAGACGT500.002789200733.9986273
GGTATCA57450.033.0795671
AACGCAG129150.030.8659636
ATCAACG129700.030.8333743
TCAACGC130350.030.7122254
CAACGCA132600.030.1590395
GTCGACA1003.094341E-629.74883
TAACTCG600.00680610828.332195
GTCGAGG17350.028.1688928
ACAGGTA4700.028.0418191
ACAGGAG3250.027.4712431
ACGCAGA145600.027.3787047
CGCAGAG146350.027.2093568
CAGCTAG1255.699985E-727.1989029
Produced by FastQC (version 0.11.5)