FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2149889
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46470.21615069429165878No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT43050.20024289626115582No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC42380.19712645629611578No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA36270.1687063843761236No Hit
GTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTC32550.15140316546575194No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT31410.14610056612225097No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA28140.13089047853168234No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTC28060.1305183662970507No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26780.1245645705429443No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA25830.12014573775669349No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT25720.11963408343407496No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCT25640.11926197119944333No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAA23740.11042430562694167No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT23560.10958705309902046No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTT22290.10367977137424306No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAAT21720.10102847170249254No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA128000.037.7998431
AACGCAG141850.034.1839336
TGGTATC39050.034.1723142
TCAACGC150250.032.3293884
ATCAACG152800.031.9289233
GTCGAGG14250.031.9120628
GTGGTAT41200.031.6811
CAACGCA154450.031.4777625
GACAGGT6850.031.0339571
TATCAAC156800.030.9246882
ACGCAGA159100.030.4783427
CGCAGAG159450.030.4121488
TTGTACG3000.028.3318233
GCAGAGT171900.028.2609399
ATTAGAG18200.027.0864683
GGCACCG17700.026.8943469
GGTATCA76000.026.2931121
AGAGGCA18050.026.134346
GCTTGTA12550.025.0695041
AGGCACC18700.024.9998288
Produced by FastQC (version 0.11.5)