FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414415

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414415
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2149889
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGC39430.18340481764407374No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTT35040.16298515876866201No Hit
GTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACAGGGAGG32400.15070545502581761No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAATAACTTG29840.13879786351760487No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29300.13628610593384125No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGC26460.12307612160441772No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTCTAATACTGGTG24250.11279652112271843No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAA23700.11023824950962585No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTT23210.107959062072507No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAGGGATAACAG22750.10581941672337503No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTAAGATTTGCCG22460.1044705098728353No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATG22050.10256343467034809No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC42550.031.1336962
GTGGTAT44700.030.01631
GACAGGT4850.027.7778871
GCGTCCG400.00786809727.50467913
GGACGGC400.00788151227.49507719
GTATCAA145150.025.874891
AGAGTAC110950.024.31857711
CAGAGTA112350.024.23530610
AACGCAG161000.023.1657966
GCACCGC11550.022.62184310
CCGGTCG1103.290952E-722.4991159
TATCAAC167600.022.400612
TCAACGC166900.022.163154
ATCAACG168400.022.0310123
ACAGGTA7000.021.995551
CAACGCA169500.021.9042515
GCGTCCA650.00324139521.1530159
ACGCAGA178000.020.938387
CGCAGAG178200.020.7958438
CGCGGTA12700.020.13372443
Produced by FastQC (version 0.11.5)