FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414416

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414416
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences811801
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT30260.3727514501706699No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT21440.2641041338948831No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14470.17824565379939172No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10770.1326679814388009No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT8150.047.4634481
TGGTATC8150.046.9331972
GTATCAA29850.043.0068971
TATCAAC33900.038.1126482
GGTATCA15950.035.712451
ATCAACG36600.035.066653
TCAACGC36900.035.0119064
CAACGCA37150.035.005085
AACGCAG38250.033.99846
ACGCAGA40800.031.975697
CGCAGAG42000.031.064018
GCAGAGT44850.029.2795589
CAGAGTA31850.024.2845710-11
GAGTACG35900.021.54494512-13
AGAGTAC30850.021.42119410-11
TATTAGA1656.3694424E-620.606362
GTACGGG38700.019.60118714-15
AGTACGG33750.019.517612-13
CTGTCGC1559.19214E-519.1926429
TCTGTCG1358.0314156E-418.8879998
Produced by FastQC (version 0.11.5)