FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414417

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414417
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences811801
Sequences flagged as poor quality0
Sequence length61
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG59140.7285036603798222No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT6100.035.194811
TGGTATC6350.033.780022
GTATCAA27900.028.6093251
GGTATCA13400.028.3457621
GCCGTAT400.00786413427.50548739
TAACGAT400.007866527.5037951
TTCGACC400.007866527.5037918
TATCAAC31050.025.5961862
TTACTCG651.0916636E-425.374044
TCAACGC34000.023.2844124
CAACGCA34150.023.2640655
ATCAACG34250.023.1173023
AGAGTAC30500.022.80618711
AACGCAG34750.022.7832746
CAGAGTA30850.022.54744510
GGGTTAC1103.2567914E-722.5197561
ACGCAGA36500.021.5402957
CGCAGAG36900.021.3067978
CGTACCC650.003244456821.1476383
AGTACGG33550.020.82125913
Produced by FastQC (version 0.11.5)