FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414419

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414419
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1326642
Sequences flagged as poor quality0
Sequence length61
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22390.16877198219263373No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC25300.033.5725442
GTGGTAT25750.033.4317441
GTATCAA79100.024.3743761
AGAGTAC62300.022.11695111
CAGAGTA64000.021.5294710
TATCAAC88600.021.5003622
GGTATCA44550.021.4226781
AACGCAG92000.020.6436926
TCAACGC91750.020.610854
ATCAACG92700.020.518233
CAACGCA93700.020.211255
ATAATAC2550.019.401973
ACGCAGA103050.018.3781227
GGCACCG1805.7934813E-918.3323789
CGCAGAG104400.018.091918
AGTACGG78000.017.52482813
GAGTACG79700.017.39258612
GCAGAGT109150.017.333049
TTAACGG1606.2156505E-717.18919835
GTCGAGG11200.017.1833658
Produced by FastQC (version 0.11.5)