FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414422

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414422
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences564853
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26410.46755527544334546No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT23520.4163915213338692No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16090.28485287322542324No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT13620.24112468199690892No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10780.19084611394469003No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT10350.1832335138522766No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAA8750.1549075600200406No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA7170.12693568061070756No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC6770.11985419215264856No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6620.11719863398087643No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT6350.11241862927168662No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6290.11135640600297776No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA6250.11064825715717186No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT6170.10923195946556007No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6160.1090549222541086No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATG5930.10498306639072467No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGA5700.10091121052734073No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTACG1600.045.165281
GTATCAA66800.035.063151
AACGCAG69750.034.0586976
GACAGGC1008.0392056E-834.00681
GGTACGG1800.033.0533873
TATCAAC75450.031.5983242
ATACGGG1351.0131771E-931.4877761
TGGTATC23900.031.1172052
GTCTAAG550.004441763730.9152721
CTTGTAC1659.094947E-1230.9070642
TCAACGC79900.029.9448434
ATCAACG80150.029.7984223
GTCGAGG9300.029.704988
GGCACCG2300.029.5658979
ACGCAGA80650.029.4555997
CAACGCA81800.029.3012565
CGCAGAG81550.029.1331028
GTGGTAT24950.028.6229571
TTGTACG755.904165E-428.3314763
GTACGGC600.006804876528.3314743
Produced by FastQC (version 0.11.5)