FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414423

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414423
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences564853
Sequences flagged as poor quality0
Sequence length61
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAA9190.16269719732390553No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8570.15172089021391408No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA6950.12304086195877512No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT6080.1076386245624968No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT6080.1076386245624968No Hit
GTATCAACGCAGAGTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCT5740.10161935937314664No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAGGT1700.030.7424741
GGCCGCT553.494831E-529.9910666
AGTTCCG606.321167E-527.49424432
AGTATCC400.00788124627.491814
CTTGAGT1256.002665E-1126.3921362
CGGGAGC1156.6211214E-1026.30350117
CGGGCCG759.763511E-625.6590234
GCTTGTC651.08627755E-425.3905431
GGGCCGC651.0908636E-425.3748075
CTGCTTA1750.025.14204818
AGAGTAC50050.025.10908111
CAGAGTA50700.025.05836310
TGAGTAC1001.3110184E-724.742634
GTATCAA78550.024.4073521
GTGGTAT26350.024.1137891
TGGTATC26550.024.0229742
TGCTTAA1750.023.56649619
ACAGGTA2500.023.1053941
AACGCAG82450.022.9737516
CGCTGTG600.002035683722.91399
Produced by FastQC (version 0.11.5)