FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414424

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414424
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1136550
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30090.26474858123267786No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT26260.2310501077823237No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19370.1704280497998328No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT13410.11798864986142271No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11680.10276714618802517No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC14600.051.5232772
GTGGTAT15500.048.8165361
GTATCAA59600.042.0095141
TATCAAC65900.038.0495642
TCAACGC70250.035.693474
ATCAACG70500.035.627183
GGTATCA31300.035.3108061
CAACGCA72100.034.8955045
AACGCAG74300.033.8622636
ACGCAGA82750.030.4044257
CGCAGAG83350.030.1855588
GTCGAGG9800.029.055738
GCAGAGT88050.028.6225579
TCGAGGT10250.026.536239
CAGAGTA52700.024.63784810-11
CTATGCG1102.0160801E-423.1815079
AGTCGAG13700.022.9558967
CCGTGCG950.002343274422.368129
GAGTACG57850.022.2975712-13
GACAGGT7500.022.1045651
Produced by FastQC (version 0.11.5)