FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414432

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414432
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1463002
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT25050.1712232792573079No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23680.1618589721681857No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14870.10164032585054565No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT18900.041.6252021
TGGTATC18900.041.1498682
GTATCAA56450.039.3989071
TATCAAC65100.034.1428572
TCAACGC69650.031.9124164
ATCAACG70900.031.5296153
CAACGCA71300.031.3527325
AACGCAG71850.031.0525196
GGTATCA32150.030.686931
ACGCAGA79650.028.0115957
CGCAGAG80050.027.9247118
GCAGAGT89400.024.9091019
AGCGTCA3650.024.4515063
GTCGAGG9200.023.0965188
CAGAGTA53550.022.34153210-11
AGAGTAC51300.020.13181310-11
ACGGGAC9650.018.4969923
AGTACGG64050.018.3797572
GAGTACG65050.018.0978312-13
TCGAGGT11450.017.8155849
Produced by FastQC (version 0.11.5)