FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1463002
Sequences flagged as poor quality0
Sequence length61
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23120.15803122620474888No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC18500.035.5081252
GTGGTAT19500.033.701021
GTATCAA69500.027.3779561
TATCAAC77250.024.3365612
AGAGTAC55000.023.80279711
CAGAGTA56400.023.4557710
GGTATCA33600.023.2412151
ATCAACG81800.022.982093
TCAACGC81750.022.8952884
AACGCAG83250.022.5157746
CAACGCA83800.022.3679965
ACGCAGA93500.020.0201267
CGCAGAG94950.019.7460428
TACCGCG856.699829E-419.4054188
AGTACGG67950.019.22595413
GAGTACG68700.018.93599512
GCAGAGT100050.018.7446259
TACGGGT14400.018.14449515
ACGGGTC4600.017.93627516
GTCGAGG10150.017.6050788
Produced by FastQC (version 0.11.5)