FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414434

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414434
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2206065
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67730.3070172456387278No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT57350.2599651415529461No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40410.18317683295823106No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA32580.14768377178369632No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT30950.14029505023650707No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26580.12048602375723291No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC26160.11858218139538046No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT24600.11151076690849998No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT23170.10502863696219286No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCA22470.10185556635910548No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGA22430.10167424803892904No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT22090.10013304231742946No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC32950.050.428682
GTGGTAT36500.046.128081
GTATCAA138150.040.4396631
TATCAAC154500.036.720542
TCAACGC164450.034.2403564
ATCAACG167000.033.92113
AACGCAG166750.033.742596
CAACGCA168150.033.51225
GGTATCA71800.033.1017071
GTCGAGG22250.031.1331968
ACGCAGA189200.029.6938557
CGCAGAG189100.029.687768
GACAGGT9100.028.9676551
GCAGAGT197100.028.4625059
TCGAGGT24950.026.9136359
CAGAGTA113800.024.87325110-11
AGTCGAG33850.023.6023627
AGAGTAC110900.021.42302710-11
GAGTACG132850.021.14683512-13
GTACGGG151750.018.23277914-15
Produced by FastQC (version 0.11.5)