FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414435

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414435
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2206065
Sequences flagged as poor quality0
Sequence length61
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44440.20144465371600564No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT27960.12674150580331947No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT23810.10792973008501563No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC39150.037.1451032
GTGGTAT41350.035.3794941
GTATCAA163950.027.0544341
AGAGTAC119350.025.16371711
CAGAGTA122300.024.71359810
TATCAAC182100.024.2143082
GGTATCA81550.023.7053761
TCAACGC195700.022.473334
ATCAACG196800.022.4320283
AACGCAG197950.022.3150886
CAACGCA198700.022.1616925
AGTACGG140950.021.17089713
GAGTACG142850.020.96632212
CGCAGAG222500.019.7965228
ACGCAGA223350.019.7914547
GCAGAGT229500.019.20739
TACGGGG48000.018.39239915
GTACGGG161150.018.1075514
TACGGGT25750.018.05028515
ACGGGAA24200.017.3880416
Produced by FastQC (version 0.11.5)