FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414444

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414444
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences741793
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21890.2950958016589534No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT18980.2558665288025096No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13060.17605989811173736No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT10820.14586279460712084No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9110.12281054148529306No Hit
GGGAATGAGAACGGAAAATCTCACTAAGGAAGACGAAGTCGATGGAGCAA8820.11890109504942754No Hit
GTATCAACGCAGAGTACGGGATCGACACAGGGAATGAGAACGGAAAATCT8000.10784679823077328No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT10850.054.504191
TGGTATC10900.054.1956562
GTATCAA42850.039.4170881
GGTATCA23550.036.492641
TATCAAC47600.036.070372
TCAACGC50750.033.747784
CAACGCA51150.033.4838645
ATCAACG51150.033.4838643
AGCGTCA1306.7848305E-1032.691353
GACAGGT3550.032.3578951
AACGCAG53000.032.315096
CGCAGAG59100.029.05168
ACGCAGA59500.028.7848687
GCAGAGT61150.028.0081739
CAGAGTA33750.025.68986710-11
CGTAGAC850.001226354224.9992683
GCAGCGT1551.3324825E-724.7033391
AACGTCG950.002342729122.3677657
CCTATAC1903.8744474E-822.3677653
GAGTACG39200.022.11893512-13
Produced by FastQC (version 0.11.5)