FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414445

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414445
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences741793
Sequences flagged as poor quality0
Sequence length61
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16660.22459095731558534No Hit
GTATCAACGCAGAGTACGGGATCGACACAGGGAATGAGAACGGAAAATCTCACTAAGGAAG7720.10407216029269621No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC13950.041.760252
GTGGTAT14300.040.9441071
TAAGACG300.001933045536.6362424
GTATCAA52600.027.3315221
AGAGTAC37000.025.79522311
CAGAGTA38500.025.21886410
CGGGATA2650.024.90850317
TATCAAC57500.024.8506012
ATCAACG60750.023.3838733
TCAACGC60400.023.3828964
CAACGCA61350.023.0208155
GGTATCA26400.023.001733
ACTCGTT600.002040693522.90537331
AACGCAG63000.022.3742756
AGTACGG43300.021.85154313
ACGGGAT9950.021.83813716
GAGTACG43950.021.59095212
ACCGCAT650.003246682521.1448448
CGTACTG650.003254308821.1362925
CGCAGAG70200.020.126768
Produced by FastQC (version 0.11.5)