FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414446

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414446
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1624124
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65030.40040046203368707No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT51380.3163551551482522No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37980.23384913959771544No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT26880.16550460432824093No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24450.15054269255303168No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT18230.11224512414076758No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16630.1023936595974199No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT25400.057.419481
TGGTATC26600.055.599272
GTATCAA98350.041.8936541
TATCAAC111200.037.4153142
GGTATCA48450.036.684391
TCAACGC118700.034.9438324
ATCAACG119500.034.781033
CAACGCA120300.034.443755
AACGCAG125200.033.16366
CGCAGAG139900.029.6494718
ACGCAGA141200.029.4065937
GTCGAGG17500.029.1425848
GCAGAGT148300.028.1142229
GACAGGT8550.027.8497121
TCGAGGT19100.024.9219979
CAGAGTA77100.024.28304310-11
CGTACGA552.0171466E-423.181634-35
AGTCGAG26200.022.5474977
AGAGTAC71350.022.42758810-11
GAGTACG87550.021.26324712-13
Produced by FastQC (version 0.11.5)