FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1624124
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33430.2058340373025705No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT31150.042.828291
TGGTATC31750.041.7410162
GTATCAA120600.026.5948941
AGAGTAC75250.025.10750811
CAGAGTA79250.024.18727310
TATCAAC133700.023.9582042
GGTATCA60750.023.3045333
ACGCTAG600.00203423222.91987850
ATCAACG143500.022.2795943
TCAACGC143650.022.2180584
CAACGCA145750.021.9545175
AACGCAG148200.021.6107866
AGTACGG87450.021.57335313
GAGTACG89500.021.07921812
CGGACGT1055.516382E-620.95402536
ACGGGTA2950.020.5088216
CGGGGAA7650.020.4905417
GACAGGT7000.020.040991
TACGGGG36000.020.01422515
ACGGGAT17950.019.91676916
Produced by FastQC (version 0.11.5)