FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414453
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences721167
Sequences flagged as poor quality0
Sequence length61
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG61190.8484858569513025No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC6650.035.132472
GTGGTAT6550.034.851331
GTATCAA31250.030.6275141
TATCAAC34650.027.6843032
GCGTCGA400.00786947527.5012520
AGAGTAC32650.026.6149811
TCAACGC36050.026.532944
GGTATCA13850.026.4109761
CAGAGTA33400.026.18019110
CAACGCA36650.026.1753795
ATCAACG36950.026.0372523
AACGCAG38450.025.0232286
CGCAGAG38800.024.8718058
ACGCAGA39550.024.398467
GCAGAGT40500.023.8973459
AGTACGG37450.022.98502213
GAGTACG38100.022.73567612
CGGGATA1705.456968E-1222.64808717
TACGGGT3700.021.55503315
CGGGAAT903.991569E-521.3898617
Produced by FastQC (version 0.11.5)