FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1979623
Sequences flagged as poor quality0
Sequence length61
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40840.20630190697925815No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT27430.13856173624978088No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT22750.11492087129721164No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC20600.10406021752626636No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC35750.039.3652572
GTGGTAT37050.038.2191351
GTATCAA136150.026.8593731
AGAGTAC95600.025.83325411
CAGAGTA97950.025.32577310
TATCAAC151800.024.1004682
ATCAACG160500.022.6222633
TCAACGC160800.022.495164
AACGCAG164450.022.0961596
GGTATCA68750.022.0364061
CAACGCA164450.021.9958745
AGTACGG112850.021.83569713
GAGTACG114600.021.50225412
ACGCAGA185700.019.5262077
CGCAGAG185800.019.475748
TACGGGG42300.019.43978515
GTACGGG127800.018.91553714
GCAGAGT192450.018.8322949
TACGGGT17000.018.11881315
ACGGGAT16100.018.10629816
Produced by FastQC (version 0.11.5)