FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences787197
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13760.1747974141161615No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAA7950.10099123853368344No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGCAC300.001927899536.65641444
GTGGTAT22050.031.1902451
TGGTATC21850.030.5691432
GTATCAA68950.025.6944271
AGAGTAC48250.024.68126711
CAGAGTA49150.024.50910610
TAGCACG801.6100861E-524.057320
CGGGGCC701.798679E-423.57382817
CCTTCGC952.3187913E-623.15289120
CGGCTCC600.002032255522.92191348
TATCAAC77150.022.766352
GCTTGTA3800.022.4422021
AACGCAG77650.022.3719626
GGTACCC2850.022.1952446
ATCAACG79900.021.809383
TCAACGC80550.021.5296554
GTCGAGG8500.021.3469728
CAACGCA81700.021.2602465
CAGGTCT650.003232171821.161381
TAGCCGG650.003242914321.14927530
Produced by FastQC (version 0.11.5)