FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1025601
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT60630.5911655702363784No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT30440.29680158268176415No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20730.20212538794326448No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13440.13104511403557526No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT11880.11583452044216025Illumina PCR Primer Index 10 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT10600.052.1308981
TGGTATC10650.050.676422
GTATCAA36800.042.968761
TATCAAC41000.038.661332
TCAACGC43100.037.07344
ATCAACG43850.036.1485633
CAACGCA44200.035.862325
AACGCAG45550.034.892746
GGTATCA19800.033.9195141
ACGCAGA51250.031.0918717
CGCAGAG52750.030.2092178
GCAGAGT55500.028.7123629
CAGAGTA36250.023.91403210-11
GTACTAG1553.7028276E-621.9389551
TACTAGA1404.203627E-521.2481842
GAGTACG40550.021.1685512-13
AGAGTAC34300.020.37991710-11
GCTTGTA2853.7107384E-1019.3890361
CTACGTT1100.00548166319.316534
ACGGGAT8300.019.19829416-17
Produced by FastQC (version 0.11.5)