FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414459

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414459
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1025601
Sequences flagged as poor quality0
Sequence length61
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG115031.121586269904183No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT7450.034.7044831
TGGTATC7750.032.9836432
GTATCAA34450.029.3814071
TATACCG400.007895566527.483695
GGTATCA16500.026.8383561
TATCAAC38900.026.4264852
AACGCAG41250.024.9197626
TCAACGC40700.024.9164314
AGAGTAC39750.024.6967111
CAACGCA41300.024.688745
ATCAACG42000.024.1452083
TACGCGA801.6075033E-524.06348229
ACGCAGA43700.023.5238067
CAGAGTA42100.023.37890810
CGCAGAG44350.022.9953528
GCAGAGT46100.022.1245849
AGTACGG44500.021.69401413
GAGTACG45450.021.23745312
TCGTAGA956.08194E-520.2531152
AATACCG1501.4406396E-820.1547075
Produced by FastQC (version 0.11.5)