FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414460

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414460
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences932889
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT51950.5568722538265539No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33880.3631728962395312No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21390.22928772876515857No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT17560.18823246924339337No Hit
GACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCGCAA12400.13292042247255567No Hit
GTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCT11510.1233801663434771No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9370.10044067407805216No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT14850.059.5516051
TGGTATC15200.058.1647532
GTATCAA50200.040.314361
TATCAAC55050.036.7527432
TCAACGC58400.034.642644
GGTATCA26150.034.294753
CAACGCA59650.033.9166835
ATCAACG59900.033.7751273
AACGCAG61500.032.8964236
ACGCAGA68200.029.8468277
CGCAGAG69700.029.2044988
GCAGAGT73100.027.788029
CAGAGTA38600.026.58746110-11
GTACGGA1457.035851E-826.3894961
AGAGTAC38300.023.13419210-11
GAGTACG44800.022.76566112-13
GCTTGTA3100.021.9439531
GTATATA1754.3277214E-721.8655821
GTACGGG47700.020.8915914-15
AGTACGG42600.020.69929512-13
Produced by FastQC (version 0.11.5)