FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414465

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414465
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1027450
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20810.20254026959949392No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT18750.043.2737921
TGGTATC18700.042.7761652
GTATCAA74550.027.7074471
ATCCGGT507.0286397E-427.50185216
CAGAGTA51900.024.9571110
AGAGTAC51100.024.81086711
TATCAAC83650.024.7445472
GGTATCA36600.023.1312433
ATCAACG89250.023.0984253
TCAACGC90200.022.855154
CAACGCA91200.022.6648245
AACGCAG94050.022.2724386
GACAGGT3650.021.8529151
AGTACGG58800.021.37473713
CCGTGCT650.003239169321.15423810
GAGTACG59800.021.1092812
CCGAATT1701.2551027E-1021.03082714
ACGCAGA106600.019.6512647
TACGGGA31800.019.63182415
CGCAGAG107550.019.4540188
Produced by FastQC (version 0.11.5)