FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414469

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414469
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1811337
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGC26280.14508619875815487No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25920.14309871658338566No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTT24000.132498811651283No Hit
GTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACAGGGAGG22170.12239577726287268No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAA21440.11836560507514615No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAATAACTTG20780.11472188775473588No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC35100.033.046182
GTGGTAT36600.032.154851
GTATCAA121950.026.1327721
GCTTGTA7700.025.354291
GCACCGC7950.024.21579610
AGAGTAC94850.023.80529811
CAGAGTA95550.023.65968310
CCGCTCG701.7991822E-423.57528722
AACGCAG135450.023.2939346
TATCAAC139150.022.8542022
ATCAACG142000.022.2775233
GCTTGTC2350.022.231541
TCAACGC141850.022.2229654
CAACGCA143300.022.0556375
GACCGCT752.87573E-421.99752420
ACGCAGA151350.020.8485267
GCCCAGT9000.020.77601219
AGACAGG4900.020.762971
CGCAGAG152550.020.6536248
GACAGGT6050.020.4522441
Produced by FastQC (version 0.11.5)