FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414471

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414471
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1068757
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20630.19302797548928333No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAA16720.15644341978578855No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGC12560.11751969811659713No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT11870.11106360005127451No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT11740.10984723374911227No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC10870.10170693618848813No Hit
GTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACAGGGAGG10800.10105196971809308No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGATATTTGCCGCCT10720.10030343660907017No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC26600.033.175812
GTGGTAT27600.032.1850551
GTATCAA93100.026.1724781
GCACCGC3950.025.76236510
CAGAGTA72900.024.14535510
AGAGTAC72550.024.07229211
AGGCACC4350.023.3857488
AGTCCGG952.313489E-623.16047754
ATTAGAG4750.023.1485563
TATCAAC105500.023.1136912
AACGCAG107300.022.5711676
GTCGGCT752.8694005E-422.00348352
TAACGGC4750.022.00142536
ATCAACG110550.021.9811823
TCAACGC110600.021.9215374
GGCACCG4700.021.648319
CAACGCA113100.021.484585
ACCGCCT5050.021.23999212
GGCCGCG4950.021.11247640
TGCCCAG5000.020.90037318
Produced by FastQC (version 0.11.5)