FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences679225
Sequences flagged as poor quality0
Sequence length91
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47570.7003570245500387No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT41250.6073098016121316No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29480.4340240715521366No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA22760.33508778387132393No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT22360.3291987191284184No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC20220.2976922227538739No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT18160.26736353932791046No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18140.2670690860907652No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGA16340.2405682947476904No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT15980.2352681364790754No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCA15550.22893739188045198No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATG15460.22761235231329824No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT14660.21583422282748724No Hit
ATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAGTACCC14340.21112297103316277No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATA13670.20125878758879603No Hit
GTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAG12630.1859472192572417No Hit
CTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAA12620.1857999926386691No Hit
GCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCTGAATGGGG12110.17829143509146456No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAA12090.17799698185431925No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12080.17784975523574661No Hit
CTTAATGGGCTGATCCCCGTTCGCTCGCCGCTACTGAGGGAATCTCAATT11720.17254959696713165No Hit
GTATCAACGCAGAGTACGGGGTCAAGTAATTAAGTGCATGTGGTGGATGC11470.1688689315028157No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11260.1657771725127903No Hit
GTATCAACGCAGAGTACGGGACTATCACCCTCTACGGTAGCACTTCCCAG11250.16562994589421767No Hit
CCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTT11050.16268541352276492No Hit
CATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGCTACTGAGGGAAT11030.16239096028561964No Hit
GGGTACTGAGATGTTTCACTTCCCCTCGTTCGCCTCGCATGACTATGTAT11010.16209650704847436No Hit
GTACTGAGATGTTTCACTTCCCCTCGTTCGCCTCGCATGACTATGTATTC10720.15782693510986787No Hit
ATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCA10610.15620744230556885No Hit
CCGCTACTGAGGGAATCTCAATTGATTTCTTTTCCTAAGGGTACTGAGAT10430.15355736317126137No Hit
CGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGC10310.15179064374838971No Hit
GGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGAGGGGAA10280.1513489638926718No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10220.15046560418123597No Hit
GCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCTG10000.1472266185726379No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9870.14531267253119365No Hit
GCAAATATCCTGTGATCCGGAGATTTCTGAATGGGGAAACCCACTTACCA9810.14442931281975782No Hit
GTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCG9720.14310427325260408No Hit
GTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAGCTTCGGGGAGGCG9580.14104310059258712No Hit
TCTCAGTACCCTTAGGAAAAGAAATCAATTGAGATTCCCTCAGTAGCGGC9290.13677352865398062No Hit
GTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAGTACCCTTAGGAA8980.13220950347822888No Hit
GGGAATGAGAACGGAAAATCTCACTAAGGAAGACGAAGTCGATGGAGCAA8920.13132614376679302No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8880.13073723729250247No Hit
CTACTGAGGGAATCTCAATTGATTTCTTTTCCTAAGGGTACTGAGATGTT8480.12484817254959696No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8160.12013692075527255No Hit
TCGCATGACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGT8150.11998969413669991No Hit
CCCACTTACCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAG7550.11115609702234162No Hit
GATGAATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAG7370.10850601788803416No Hit
AATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACG7360.10835879126946152No Hit
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7330.10791711141374362No Hit
GTGGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTT7050.10379476609370973No Hit
GTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAG7030.10350031285656447No Hit
GTATCAACGCAGAGTACGGGTCAAGTAATTAAGTGCATGTGGTGGATGCC6950.10232249990798337No Hit
CCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAGCTTCG6930.10202804667083809No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC21350.050.5565032
GTGGTAT21550.049.528531
GTATCAA84000.039.68861
GACAGGT2450.038.184371
CGTCAGA1151.8189894E-1036.9524575
TATCAAC94700.035.854032
GGTATCA42750.035.212441
TAGTACG500.00278064134.01881
ATCAACG104600.032.5012053
AACGCAG103700.032.4554446
TCAACGC105000.032.4178624
CAACGCA107050.031.836765
GTCCTAG550.00443472730.9261821
CAGACGC1401.4897523E-930.373931
AAGTACG1452.1682354E-929.3265511
ACGCAGA117550.028.7037777
CGCAGAG118350.028.5139488
ACAGGTA3300.028.3489991
CGAGTGT300.006808445328.33125916-17
GCAGAGT122300.027.7340669
Produced by FastQC (version 0.11.5)