FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414477

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414477
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1144921
Sequences flagged as poor quality0
Sequence length61
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG59480.5195118265801746No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT18250.15939964416758887No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC14690.12830579577106194No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT14630.12778174214640137No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCT13340.11651458921619919No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGATATTTGCCGCCT12920.11284621384357524No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCC12290.10734365078463928No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAGCTTCGGGGA12200.10655757034764843No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAA11920.10411198676589914No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA38250.031.365541
GTGGTAT8050.030.764111
TGGTATC8200.029.5160162
TATCAAC44250.027.286022
GGTATCA16750.026.6132491
ATCAACG45850.026.1458823
TCAACGC45650.026.139974
AACGCAG46450.025.571386
CAACGCA46500.025.5427675
AGAGTAC46050.024.54071611
ACGCAGA48550.024.3520417
CAGAGTA47000.024.33719810
CGCAGAG49750.023.819928
GCAGAGT53000.022.412129
AGTACGG51800.021.8185113
GAGTACG54300.020.76333412
GATCGAC1251.1238171E-619.79910320
TACGGGG22800.019.41882915
GTACGGA1702.8485374E-919.41088514
ACGGGGT9200.019.13040416
Produced by FastQC (version 0.11.5)