FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414479

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414479
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences535682
Sequences flagged as poor quality0
Sequence length61
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7180.13403474449393485No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC13050.038.5283932
GTGGTAT13200.037.6918751
ATACCGC507.0641894E-427.4751646
GTATCAA38800.024.2999571
GGTATCA22050.023.0517253
GGATGCG600.002031545222.92165211
TACGCAC600.002031545222.92165241
AGAGTAC27700.022.54100411
CAGAGTA28250.022.10215210
TATCAAC43700.021.50232
CTATCGG650.003233433721.1584512
GATGCGT650.003233433721.1584512
GATCGAC650.003245723421.14461320
TTACCCT1108.309018E-619.9800724
AACGCAG46950.019.8968166
TCAACGC46950.019.8364454
ATCAACG47400.019.823853
ATGCGTA700.00496637119.64713113
TTAAACG700.00496637119.64713122
CTATATC700.005001380619.6232854
Produced by FastQC (version 0.11.5)