FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414483

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414483
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences785325
Sequences flagged as poor quality0
Sequence length61
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT10620.13523063699742144No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGATATTTGCCGCCT8490.10810810810810811No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT8480.10798077229172635No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCT8440.10747142902619936No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30550.031.9725861
GGTATCA12850.028.6920971
GTGGTAT5550.028.2580721
TATCAAC35100.027.6571122
CAACGCA36950.026.3367445
TCAACGC36800.026.2913464
ATCAACG36900.026.2234343
AACGCAG36900.026.1489376
TGGTATC6000.026.1253932
ACGCAGA37750.025.487337
AGAGTAC37600.025.37613911
CGCAGAG38500.024.921018
CGGGATA2100.024.88291217
CAGAGTA38550.024.74763310
ACGGGAA4950.024.4463716
GCAGAGT40400.023.6824429
AGTACGG40950.023.03600313
GAGTACG42100.022.46778512
TACGGGT5100.022.10958515
ACGGGTC3000.021.08499316
Produced by FastQC (version 0.11.5)