FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414485

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414485
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1950396
Sequences flagged as poor quality0
Sequence length61
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30020.15391746086435779No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC24150.036.535672
GTGGTAT24950.036.032591
GTATCAA79700.025.181571
AGAGTAC59750.023.6603811
ACGGGGC4900.023.57489216
CAGAGTA63400.022.64528310
GGTATCA42300.022.0332011
TATCAAC91000.021.8688682
ATCAACG91950.021.52283
TCAACGC92300.021.4114074
CAACGCA93550.021.1840745
AGTACGG67700.020.80069213
AACGCAG96050.020.5182276
GAGTACG69050.020.2745212
GTCGCCG700.004973763619.6452428
CGCCGTG856.6922815E-419.4091430
TACGGGG24550.019.38166615
CGCAGAG106100.018.5262138
GTACGGG75650.018.39662214
ACGCAGA107300.018.3935267
Produced by FastQC (version 0.11.5)