FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414486

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414486
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences639585
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29490.46108023171275125No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT22080.3452238560941861No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17050.2665791098915703No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT11790.18433828185464013No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11170.1746444960404012No Hit
GGGAATGAGAACGGAAAATCTCACTAAGGAAGACGAAGTCGATGGAGCAA8910.13930908323365934No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT8690.13586935278344553No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8100.12664462112150848No Hit
GTATCAACGCAGAGTACGGGATCGACACAGGGAATGAGAACGGAAAATCT7750.12117232267798651No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6630.10366096765871619No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6430.10053393997670364No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC10050.060.049532
GTGGTAT10400.058.0649531
GTATCAA42800.041.4334751
GGTATCA21100.039.301691
TATCAAC48000.038.0727772
TCAACGC50750.036.1772084
ATCAACG50600.036.1164673
CAACGCA52100.035.4029435
AACGCAG53650.034.2190066
GTCGAGG9100.029.8876658
CGCAGAG62300.029.6725318
AGTCGAG12000.029.3934277
ACGCAGA62950.029.2986357
GCAGAGT65350.028.4177199
TCGAGGT9250.027.105899
CAGAGTA31850.026.68541310-11
CACACCG808.6074375E-426.5624034
AGAGTAC30550.023.23050510-11
CGGGTAC652.3700979E-522.8863216-17
GAGTACG37600.022.55066512-13
Produced by FastQC (version 0.11.5)