FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414489

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414489
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences740734
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20140.2718924742215154No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT13100.044.7100071
TGGTATC13700.042.5425722
CGGCTAT400.00786418627.50513552
CGTGCTC400.00786937327.50141710
AGACCGG400.00787196827.4995619
GTATCAA49900.027.2772831
AGAGTAC30800.025.17986911
CGATCGT550.001224808325.0046747
CAGAGTA32200.024.51213510
TATCAAC55650.024.3057162
GGTATCA25600.023.6212653
CCGATCG600.002033440422.91939746
ATCAACG58950.022.8471873
TCAACGC59050.022.8084954
CAACGCA60050.022.3371245
AACGCAG62150.021.7592776
TAGCGAA650.003237706121.15493836
CTACGGC650.003241513221.1506531
GACAGGT2650.020.7529831
CGGGATA2053.6379788E-1220.1243517
Produced by FastQC (version 0.11.5)