FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414490

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414490
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21752
Sequences flagged as poor quality0
Sequence length91
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3491.604450165502023No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2641.2136815005516732No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2000.9194556822361162No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1960.901066568591394No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1450.6666053696211842No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT1290.593048915042295No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1130.5194924604634057No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1100.5057006252298639No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT1050.482714233173961No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT880.40456050018389117No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT850.3907686649503494No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT820.3769768297168076No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT790.3631849944832659No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT740.340198602427363No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA730.3356013240161824No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT700.3218094887826407No Hit
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT620.285031261493196No Hit
TATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA600.2758367046708349No Hit
ATATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA580.26664214784847373No Hit
GTTTATGGGGTTTTTGTTGTTTTGTCATTTTTATTCTTTTTTCGCTTGGT570.2620448694372931No Hit
GTGGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTT540.2482530342037514No Hit
GTATCAACGCAGAGTACGGGAAGACAAAAAAGTCAAAAAGGAAAACCTAG510.23446119897020962No Hit
GTATCAACGCAGAGTACGGGGTGGCCCAGAAGTGGAAATTTCTGCCAAGC500.22986392055902904No Hit
ACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT500.22986392055902904No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG470.2160720853254873No Hit
GTGGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA450.20687752850312613No Hit
GTATTATTTTTTTGGTTTATGTTTTTTTTCAGTTTCGGTTTCCTATTCTT450.20687752850312613No Hit
GAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT420.1930856932695844No Hit
GTATCAACGCAGAGTACGGGGAAACAAAACAAAAAGTTACAAAACAGAAA410.1884884148584038No Hit
GGGAATGAGAACGGAAAATCTCACTAAGGAAGACGAAGTCGATGGAGCAA410.1884884148584038No Hit
GTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT410.1884884148584038No Hit
ATATAATTAAGAAGCAACATAAGTAAAAGAAAAAATAAAAGTGCAGAAAA400.18389113644722324No Hit
CAACAAAACCAAGCGAAAAAAGAATAAAAATGACAAAACAACAAAAACCC400.18389113644722324No Hit
GTATCAACGCAGAGTACGGGATCGACACAGGGAATGAGAACGGAAAATCT390.17929385803604267No Hit
GTATCAACGCAGAGTACGGGCAAGGAGAAGTGAAATAAAAAAGAAAAAAA390.17929385803604267No Hit
AAACAAAAAGTTACAAAACAGAAAAATAAAAAAAACCAACAAACCAAAAA350.16090474439132035No Hit
GGTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA340.15630746598013975No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG330.15171018756895915No Hit
GTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330.15171018756895915No Hit
ATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320.1471129091577786No Hit
GTATCAACGCAGAGTACGGGAGGACAAGAAAGACGAGAAAAAAAGTTTTC320.1471129091577786No Hit
ACTCTGCGTTGATACCACTGCTTAAACGCAGAGTCGAGGTTTTTTTTTTT310.142515630746598No Hit
GCCCAGAAGTGGAAATTTCTGCCAAGCAACGTTTAAGTCGTTTGGCGGAT310.142515630746598No Hit
TATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310.142515630746598No Hit
GAGTACGGGAAGACAAAAAAGTCAAAAAGGAAAACCTAGGAAAAGAAGAA300.13791835233541744No Hit
ATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300.13791835233541744No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG290.13332107392423687No Hit
CATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGA290.13332107392423687No Hit
GTTGAGGGGTTATGAATAATTTATTAAATTTGTGGGGGATAAAATGGTGT290.13332107392423687No Hit
ACATAAGTAAAAGAAAAAATAAAAGTGCAGAAAAAGGTATAAATATAAGG280.12872379551305627No Hit
GGTATCAACGCAGAGTACGGGGAAACAAAACAAAAAGTTACAAAACAGAA280.12872379551305627No Hit
GTATCAACGCAGAGTACGGGATGCTAGAAGATGAACAAGGACGAAGAAAA280.12872379551305627No Hit
CAGTTTAACTGCTTGGTGCCAGCCAATGATCACTGCCCGCTTTCCAGTCG280.12872379551305627No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAG270.1241265171018757No Hit
TATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270.1241265171018757No Hit
GTATCAACGCAGAGTACGGGAACAAAAAAAAAAAAAAAAAAAAAAAAAAA260.11952923869069511No Hit
CAGTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTT260.11952923869069511No Hit
CTAAAAAACAATCTCAAACAATACCACAAATACCAAAAATAAACAAAAAA260.11952923869069511No Hit
ACACAGGGAATGAGAACGGAAAATCTCACTAAGGAAGACGAAGTCGATGG250.11493196027951452No Hit
CTAGAAGATGAACAAGGACGAAGAAAAAACCAATTAAACACCATTTTATC250.11493196027951452No Hit
GGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240.11033468186833395No Hit
GTATCAACGCAGAGTACGGGGGGGGGATAAATATAATTAAGAAGCAACAT240.11033468186833395No Hit
ACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240.11033468186833395No Hit
GTATCAACGCAGAGTACGGGAAACAACAAAAAGTAATAAACAACAAGCAC230.10573740345715336No Hit
GTATCAACGCAGAGTACGGGAACAAACAAAAAACAAAACAAAAAAAAAAT230.10573740345715336No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG230.10573740345715336No Hit
GTCTATTTATATATATTTTCTTTATAGTTTTTTAAATTTGAAAACTTTTT220.10114012504597279No Hit
GTTACAAAACAGAAAAATAAAAAAAACCAACAAACCAAAAAAAAAATAAA220.10114012504597279No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG220.10114012504597279No Hit
CAACAAAAAGTAATAAACAACAAGCACAACAAAAACACAAACAAAACACA220.10114012504597279No Hit
GAAGAAGGACAAAGCAAAAGAATAGGAAACCGAAACTGAAAAAAAACATA220.10114012504597279No Hit
TATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.10114012504597279No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAT150.001015941584.999991
GTGGTAT900.075.555561
TGGTATC950.071.578952
TTATGGG200.00318111163.7500043
AGGTATC200.00318111163.7500042
CAGGTAT200.00318111163.7500041
TATGGGG200.00318111163.7500044
GTATCAA5050.043.7623751
TAAATCA209.220686E-442.50000412-13
GGTATCA2150.037.5581363
TATCAAC6450.036.240312
AAATCAA250.002759734633.99999612-13
CCCCAAC250.002759734633.99999684-85
ATCAACG7550.031.5231783
TCAACGC8050.029.5652164
CAACGCA8150.029.2024545
AACGCAG8200.029.024396
ACGCAGA9600.024.7916667
CGCAGAG9600.024.7916668
GTCGAGG1401.4196576E-624.2857158
Produced by FastQC (version 0.11.5)