FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414491

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414491
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21752
Sequences flagged as poor quality0
Sequence length61
%GC32

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5562.556086796616403No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA760.3493931592497242No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT670.30801765354909894No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT620.285031261493196No Hit
GTATCAACGCAGAGTACGGGAAGACAAAAAAGTCAAAAAGGAAAACCTAGGAAAAGAAGAA580.26664214784847373No Hit
GTATCAACGCAGAGTACGGGGAAACAAAACAAAAAGTTACAAAACAGAAAAATAAAAAAAA520.23905847738139022No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT490.2252666421478485No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT430.19768297168076499No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA420.1930856932695844No Hit
GTATCAACGCAGAGTACGGGGTGGCCCAGAAGTGGAAATTTCTGCCAAGCAACGTTTAAGT420.1930856932695844No Hit
GTATTATTTTTTTGGTTTATGTTTTTTTTCAGTTTCGGTTTCCTATTCTTTTGCTTTGTCC390.17929385803604267No Hit
GTATCAACGCAGAGTACGGGAGGACAAGAAAGACGAGAAAAAAAGTTTTCAAATTTAAAAA390.17929385803604267No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT380.1746965796248621No Hit
GGGAATGAGAACGGAAAATCTCACTAAGGAAGACGAAGTCGATGGAGCAATCATCAAGGAT370.1700993012136815No Hit
GTATCAACGCAGAGTACGGGATCGACACAGGGAATGAGAACGGAAAATCTCACTAAGGAAG360.16550202280250093No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT360.16550202280250093No Hit
GTTTATGGGGTTTTTGTTGTTTTGTCATTTTTATTCTTTTTTCGCTTGGTTTTGTTGGTTT340.15630746598013975No Hit
ACTCTGCGTTGATACCACTGCTTAAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT330.15171018756895915No Hit
GTATCAACGCAGAGTACGGGCAAGGAGAAGTGAAATAAAAAAGAAAAAAATAATAAAAGAG320.1471129091577786No Hit
GTATCAACGCAGAGTACGGGATGCTAGAAGATGAACAAGGACGAAGAAAAAACCAATTAAA310.142515630746598No Hit
ATATAATTAAGAAGCAACATAAGTAAAAGAAAAAATAAAAGTGCAGAAAAAGGTATAAATA310.142515630746598No Hit
GTATCAACGCAGAGTACGGGAACAAACAAAAAACAAAACAAAAAAAAAATTAAAAAACAAA310.142515630746598No Hit
GCCCAGAAGTGGAAATTTCTGCCAAGCAACGTTTAAGTCGTTTGGCGGATTTATTACAAGC300.13791835233541744No Hit
GTATCAACGCAGAGTACGGGGGGGGGATAAATATAATTAAGAAGCAACATAAGTAAAAGAA290.13332107392423687No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290.13332107392423687No Hit
CAGTTTAACTGCTTGGTGCCAGCCAATGATCACTGCCCGCTTTCCAGTCGGGAAACCTGTC280.12872379551305627No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280.12872379551305627No Hit
GTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270.1241265171018757No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260.11952923869069511No Hit
ACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250.11493196027951452No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250.11493196027951452No Hit
GTGGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA250.11493196027951452No Hit
CATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGATCATTGGCTGG240.11033468186833395No Hit
GAGTACGGGATCGACACAGGGAATGAGAACGGAAAATCTCACTAAGGAAGACGAAGTCGAT240.11033468186833395No Hit
GGTATCAACGCAGAGTACGGGGAAACAAAACAAAAAGTTACAAAACAGAAAAATAAAAAAA240.11033468186833395No Hit
GTATCAACGCAGAGTACGGGAAACAACAAAAAGTAATAAACAACAAGCACAACAAAAACAC240.11033468186833395No Hit
GTTTTTGTTGTTTTGTCATTTTTATTCTTTTTTCGCTTGGTTTTGTTGGTTTTTTTTTTTT230.10573740345715336No Hit
GAGTACGGGAAGACAAAAAAGTCAAAAAGGAAAACCTAGGAAAAGAAGAAGGACAAAGCAA230.10573740345715336No Hit
CCCGTGACCTCCACCAAGTCCCGGGTGCGTCGCTCGCATAATCGGGCACATCGTAAGGATA220.10114012504597279No Hit
GTATCAACGCAGAGTACGGGGGGGACGAAAGATACAAAGAAATAAAAAAACCCCCAGAAAG220.10114012504597279No Hit
GCAGTAGACTGGCTGATTAAATGATTTGAATCTAAAAATAAAAAAATTGAAACTAAAGAAA220.10114012504597279No Hit
CTAGAAGATGAACAAGGACGAAGAAAAAACCAATTAAACACCATTTTATCCCCCACAAATT220.10114012504597279No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220.10114012504597279No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC1800.045.8333362
GTGGTAT1750.045.5714261
CATTTTT300.001884437636.66666813
CGGGAAC300.001884437636.66666817
TTATTCT350.00401261931.4285724
GGGAACA350.00401261931.42857218
TTTATTC350.00401261931.4285723
GGAACAA350.00401261931.42857219
TCTTTTT506.811129E-427.58
TTCGCTT400.00770730527.49999814
CGCTTGG400.00770730527.49999816
TTTTCGC400.00770730527.49999812
CTTGGTT400.00770730527.49999818
AAAAGAG400.00770730527.49999855
TCGCTTG400.00770730527.49999815
TATTCTT400.00770730527.4999985
TTTCGCT400.00770730527.49999813
GTATCAA6700.024.6268651
AGAGTAC4400.024.37511
GGTATCA3550.024.0140863
Produced by FastQC (version 0.11.5)